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HUS1 Gene

protein-coding GIFtS: 58

GC07M047735

HUS1 checkpoint homolog (S. pombe)
(Previous name: HUS1 (S. pombe) checkpoint homolog )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

		Products

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
About This Section

Aliases & Descriptions
HUS1 checkpoint homolog (S. pombe)¹ ²		checkpoint protein HUS1²
hHUS1² ³		OTTHUMP00000208365²
HUS1 (S. pombe) checkpoint homolog¹		OTTHUMP00000208366²
hus1+-like protein²		OTTHUMP00000208378²

External Ids:	HGNC: 5309¹	Entrez Gene: 3364²	Ensembl: ENSG00000136273⁷	UniProtKB: O60921³

Export aliases for HUS1 gene to outside databases

Previous GC identifers: GC07M047650 GC07M047744 GC07M047777 GC07M047969

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for HUS1:

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex

that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with

checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein

complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads

the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the

activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. (provided by

RefSeq)

UniProtKB/Swiss-Prot: HUS1_HUMAN, O60921

Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The

9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex.

Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair

(LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH

end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage

activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on

long-patch base excision repair substrates

Gene Wiki entry for HUS1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 60), Regulatory elements and Epigenetics data according to Qiagen and/or SABiosciences)
About This Section

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the HUS1 gene promoter:
         p53   RFX1   POU2F1   STAT5A   MIF-1   FOXO4   FOXO3b   FOXO3a   FOXL1   ATF-2
         Other transcription factors

Search SABiosciences Chromatin IP Primers for HUS1

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays for HUS1

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 7p13-p12 Ensembl cytogenetic band: 7p12.3 HGNC cytogenetic band: 7p13-p12

HUS1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
HUS1 gene location

GeneLoc gene densities for chromosome 7 GeneLoc Exon Structure

GeneLoc location for GC07M047735: view genomic region (about GC identifiers)

Start:	47,735,328 bp from pter
End:	48,019,178 bp from pter
Size:	283,851 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000007.13 NT_007819.17 NT_079592.2

(According to ¹UniProtKB, neXtProt, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Millipore, Sigma-Aldrich, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, and/or ProSpec,
Biochemical Assays by Millipore, Sigma-Aldrich, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Dec 2010 and Entrez Gene, Antibodies by Millipore, Sigma-Aldrich, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, and/or Epitomics)
About This Section

UniProtKB/Swiss-Prot: HUS1_HUMAN, O60921 (See protein sequence)

Recommended Name: Checkpoint protein HUS1

Size: 280 amino acids; 31691 Da

Subunit: Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex

associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC

complex. HUS1 interacts with POLB, HDAC1, FEN1, PCNA, RAD1, RAD9A and RAD9B. HUS1 does not interact with RAD17.

Interacts with DNAJC7

Subcellular location: Nucleus. Cytoplasm. Note=In discrete nuclear foci upon DNA damage. According to PubMed:14500360,

localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the

cytoplasm

PDB structures from and Proteopedia :

3A1J (3D)

3G65 (3D)

3GGR (3D)

Explore the universe of human proteins at neXtProt for HUS1: NX_O60921

REFSEQ proteins: NP_004498.1

ENSEMBL proteins:

ENSP00000397836 ENSP00000400727 ENSP00000416588 ENSP00000258774 ENSP00000387961 ENSP00000403844

ENSP00000398806 ENSP00000404348 ENSP00000405175 ENSP00000404855

Human Recombinant Proteins

	Browse Purified and Recombinant Proteins at Millipore
	Browse Human Recombinant Proteins at Sigma-Aldrich
	Browse R&D Systems for human recombinant proteins
	Enzo Life Sciences proteins for HUS1
	OriGene Purified Protein: HUS1 OriGene Protein Over-expression Lysate: HUS1
	GenScript Custom Purified and Recombinant Proteins Services for HUS1
	Novus Biologicals Protein for HUS1
	Novus Biologicals Lysates for HUS1
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins

5 Gene Ontology (GO) cellular component terms (GO ID links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IDA	10846170
GO:0005654	nucleoplasm	TAS	--
GO:0005730	nucleolus	IDA	18029348
GO:0005737	cytoplasm	IEA	--
GO:0005794	Golgi apparatus	IDA	18029348

About this table

HUS1 for ontologies About GeneDecksing

Antibodies for HUS1:

	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Sigma-Aldrich Antibodies for HUS1
	Browse R&D Systems for Antibodies
	OriGene Antibodies: HUS1
	GenScript Custom Superior Antibodies Services for HUS1
	Novus Biologicals Antibodies for HUS1
	Epitomics antibodies for HUS1

Assays for HUS1:

	Browse Kits and Assays available from Millipore
	Browse ELISAs at Sigma-Aldrich
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for HUS1
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for HUS1

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

HUS1 for domains About GeneDecksing

2 InterPro domains/families:

IPR016580 Cell_cycle_HUS1

IPR007150 Hus1

Graphical View of Domain Structure for InterPro Entry O60921

ProtoNet protein and cluster: O60921

UniProtKB/Swiss-Prot: HUS1_HUMAN, O60921

Similarity: Belongs to the HUS1 family

(According to UniProtKB, IUBMB,and/or Genatlas, Animal models from MGI Dec 24 2010,
shRNA from OriGene, Sigma-Aldrich, RNAi from Millipore, siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich, microRNA from Sigma-Aldrich, Qiagen, SABiosciences, Clones from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, Cell Lines from GenScript, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene.)
About This Section

UniProtKB/Swiss-Prot: HUS1_HUMAN, O60921

Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The

9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex.

Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair

(LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH

end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage

activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on

long-patch base excision repair substrates

Inhib. RNA:		Browse for Gene Knock-down Tools from Millipore
		Browse Nano Scale siRNA at Sigma-Aldrich Sigma-Aldrich esiRNA and siRNA for HUS1 Sigma-Aldrich shRNA Panels and shRNA for HUS1
		OriGene 29mer shRNA kits in GFP-retroviral vector: HUS1 OriGene shRNA RFP: HUS1 OriGene basic RS shRNA: HUS1 OriGene siRNA: HUS1
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of HUS1

miRNA:		Sigma-Aldrich microRNA Mimics for HUS1 Browse microRNA target validation systems at Sigma-Aldrich
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of HUS1
		Search SABiosciences for Assays for microRNAs that regulate HUS1

Gene
Editing:

Sigma-Aldrich CompoZr Knockout ZFN for HUS1

Clones:		Browse Clones for the Expression of Recombinant Proteins Available from Millipore
		Browse iPSC Reprogramming Factors at Sigma-Aldrich
		OriGene GFP tagged cDNA clones in CMV expression vector: HUS1 OriGene Myc/DDK tagged cDNA clones in CMV expression vector: HUS1 OriGene untagged cDNA clones in CMV expression vector: HUS1 OriGene 3'-UTR clone: HUS1 Browse MicroRNA Expression Plasmids
		GenScript cDNA clone with any tag delivered in your preferred vector: HUS1
		Browse Sino Biological Human cDNA Clones

Cell
Lines:

GenScript Custom overexpressing Cell Line Services for HUS1

Genatlas biochemistry entry for HUS1:

cell cycle G2 checkpoint control gene,homologous to S pombe Hos1

2 Gene Ontology (GO) molecular function terms (GO ID links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0004674	protein serine/threonine kinase activity	IEA	--
GO:0005515	protein binding	IPI	10359610

About this table

HUS1 for ontologies About GeneDecksing

Animal Models: 9 MGI mutant phenotypes (inferred from 4 alleles) (MGI details for Hus1):

cardiovascular system	cellular	embryogenesis	growth/size	hematopoietic system
lethality-prenatal/perinatal	limbs/digits/tail	nervous system	tumorigenesis

HUS1 for phenotypes About GeneDecksing

(Pathways according to Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB, Sets of similar genes according to GeneDecks, PCR Arrays from SABiosciences, Proteins Network according to SABiosciences, Sigma-Aldrich, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene).
About This Section

HUS1 for pathways About GeneDecksing

1 Millipore Pathway for HUS1

Cell cycle Role of 14-3-3 proteins in cell cycle regulation

1 Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Pathway for HUS1

Role of CHK Proteins in Cell Cycle Checkpoint Control

SABiosciences Pathway-Focused PCR Arrays including HUS1: PAHS-020A PAHS-029A

Sigma-Aldrich "Your Favorite Gene" (powered by Ingenuity) Molecular Interaction Network for HUS1

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for HUS1

5/21 Interacting proteins for HUS1 (ENSP00000258774³ O60921^{1, 2}) via UniProtKB, MINT, and/or STRING (see all 21)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
RAD1	O60671¹	STRING: ENSP00000340879 EBI-1056174,EBI-721835
RAD9A	Q99638¹	STRING: ENSP00000311360 EBI-1056174,EBI-2606224
HPRT1	P00492¹	EBI-1056174,EBI-748210
PSMA2	P25787¹	EBI-1056174,EBI-603262
YWHAZ	P63104²	MINT-3318251

About this table

5/11 Gene Ontology (GO) biological process terms (GO ID links to tree view) (see all 11):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000075	cell cycle checkpoint	TAS	--
GO:0000077	DNA damage checkpoint	IEA	--
GO:0000724	double-strand break repair via homologous recombination	IEA	--
GO:0006260	DNA replication	EXP	12791985
GO:0006281	DNA repair	IEA	--

About this table

HUS1 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

HUS1 for compounds About GeneDecksing

Browse Small Molecules at Sigma-Aldrich

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for HUS1
1 Novoseek chemical compound relationship for HUS1 gene

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
hydroxyurea	47.7	3	11077446 (1), 17327271 (1)

About this table

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 228 Homo sapiens; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Millipore,
siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene, microRNA from Sigma-Aldrich, Qiagen, SABiosciences,
Tagged/untagged cDNA clones from OriGene, Sigma-Aldrich, GenScript, Primers from OriGene and/or SABiosciences)
About This Section

Inhib. RNA:		Browse for Gene Knock-down Tools from Millipore
		Browse Nano Scale siRNA at Sigma-Aldrich Sigma-Aldrich esiRNA and siRNA for HUS1 Sigma-Aldrich shRNA Panels and shRNA for HUS1
		OriGene 29mer shRNA kits in GFP-retroviral vector: HUS1 OriGene shRNA RFP: HUS1 OriGene basic RS shRNA: HUS1 OriGene siRNA: HUS1
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of HUS1

miRNA:		Sigma-Aldrich microRNA Mimics for HUS1 Browse microRNA target validation systems at Sigma-Aldrich
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of HUS1
		Search SABiosciences for Assays for microRNAs that regulate HUS1

Clones:		Browse FLAG tag genes at Sigma-Aldrich
		OriGene GFP tagged cDNA clones in CMV expression vector: HUS1 OriGene Myc/DDK tagged cDNA clones in CMV expression vector: HUS1 OriGene untagged cDNA clones in CMV expression vector: HUS1 OriGene 3'-UTR Clone: HUS1 Browse OriGene MicroRNA Expression Plasmids
		GenScript cDNA clone with any tag delivered in your preferred vector: HUS1

Primers:		OriGene genome-wide validated SYBR primer pairs: HUS1 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay for HUS1: PPH00922A

REFSEQ mRNAs for HUS1 gene:

NM_004507.2

Additional cDNA sequence:

AJ227901.1 AK097182.1 AK290459.1 AK294117.1 BC007013.1 BT019481.1 BT019482.1 CR536552.1

CR619988.1 Y16893.1

8 DOTS entries:

DT.310408 DT.70103554 DT.92424430 DT.95259816 DT.102823648 DT.92424429 DT.91652334 DT.95362322

24/87 AceView cDNA sequences (see all 87):

NM_004507 CR536552 BQ646867 BF222727 AW132130 BX500811 CA418558 BC007013

AI167268 BU193379 CR619988 AI538328 AL554895 R95460 AA828114 AA854956

BE874516 AK097182 AA773515 BF510091 AI962021 AF110393 AA280710 BE891273

highest scoring ESTs for HUS1:

AF076844 AA280710 AA353895 AI968626 BC007013 BE543378 BE568470 BE874516 BE892098 BF185772

Unigene Cluster for HUS1:

HUS1 checkpoint homolog (S. pombe)

Hs.152983 [show with all ESTs]

Unigene Representative Sequence: CR619988

GeneLoc Exon Structure

5 Alternative Splicing Database (ASD) splice patterns (SP) for HUS1

ExUns:	1a	·	1b	^	2a	·	2b	^	3a	·	3b	^	4	^	5	^	6	^	7	^	8a	·	8b	·	8c	^	9	^	10	^	11
SP1:					-						-				-				-						-
SP2:																									-
SP3:											-
SP4:					-
SP5:

About this scheme

ECgene alternative splicing isoforms for HUS1
11 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000468868 ENST00000445133 ENST00000458191(uc011kce.1) ENST00000432325

ENST00000258774(uc003tod.1) ENST00000441837 ENST00000436444(uc003toe.1)

ENST00000446009 ENST00000442024 ENST00000433977 ENST00000432627

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 228 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Primers from OriGene and/or SABiosciences )
About This Section

HUS1 expression in normal and diseased human tissues

¹ / ² / ³

9 probe-sets matching HUS1 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
38153_at²^{, 3}	U95-A	1	1.00	1.00	0.81	0.98	AF076844	1.00	0.90	0.95	1
80446_at²^{, 3}	U95-E	1	0.88	1.00	0.95	1.12	AI968626	0.80	1.00	0.91	1
80356_r_at²^{, 3}	U95-E	1	0.44	1.00	0.63	0.93	AW007988	0.40	1.00	0.76	1
204883_s_at²^{, 3}	U133-A	1	1.00	1.00	--	--	AI968626	0.80	1.00	0.91	1
204884_s_at²^{, 3}	U133-A	1	0.91	1.00	--	--	NM_004507	0.60	1.00	0.82	1
217618_x_at²^{, 3}	U133-A	1	0.46	1.00	--	--	AW007988	0.40	1.00	0.76	1
204883_s_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--
204884_s_at²	U133Plus2	1	0.91	1.00	--	--	--	--	--	--	--
217618_x_at²	U133Plus2	1	0.46	1.00	--	--	--	--	--	--	--

About this table

HUS1 for expression About GeneDecksing

Data from Genenote (Publications) and GNF BioGPS
About these images

HUS1 gene electronic northern expression

About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.152983

Expression variation in blood from EXPOLDB for HUS1

UniProtKB/Swiss-Prot: HUS1_HUMAN, O60921

Tissue specificity: Ubiquitous

Primers:		OriGene genome-wide validated SYBR primer pairs: HUS1 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay for HUS1: PPH00922A

SABiosciences Expression via Pathway-Focused PCR Arrays including HUS1: PAHS-020A PAHS-029A

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Dec 24 2010, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for HUS1 gene from 5/14 species (see all 14)

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	HUS1¹	--	HUS1 checkpoint homolog (S. pombe)	80.23(n) 84.41(a)	606789 XM_843198.1 XP_848291.1
chimpanzee (Pan troglodytes)	HUS1¹	--	HUS1 checkpoint homolog (S. pombe)	99.64(n) 100(a)	463402 XM_519090.2 XP_519090.2
cow (Bos taurus)	HUS1¹	--	HUS1 checkpoint homolog (S. pombe)	87.98(n) 91.79(a)	514142 XM_001250856.1 XP_001250857.1
rat (Rattus norvegicus)	Hus1¹	--	HUS1 checkpoint homolog (S. pombe)	83.69(n) 86.43(a)	498411 XM_573658.2 XP_573658.2
mouse (Mus musculus)	Hus1^{1 , 5}	11 (5.74 cM)⁵	Hus1 homolog (S. pombe)^{1, 5}	83.69(n)¹ 86.07(a)¹	15574¹ NM_008316.3¹ NP_032342.1¹ AF076845⁵ AK046152⁵ (see all 12)

About this table Species with no ortholog for HUS1

ENSEMBL Gene Tree for HUS1 (if available)

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for HUS1 gene

HUS1B²

HUS1 for paralogs About GeneDecksing

(SNPs according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section

SNP ID	Valid	Chr 7 pos	Sequence	Recs	AA Chg	Type	More	Recs	Pop	Total sample
Genomic Data				Transcription Related Data				Allele Frequencies
			--	--	--		--	--
rs953572^1,2	C,F,A,	48012361(-)	`CCACAT/CACCTG`	1	--	int¹		5	NS NA EA	446
rs953594^1,2	C,F,A,	48009019(-)	`GAGGTT/AYTGTA`	1	--	int¹		7	NS MN EA NA WA	716
rs953595^1,2	C,F,A,	48009018(-)	`AGGTWT/CTGTAG`	1	--	int¹		8	NS MN EA NA WA	828
rs1056663^1,2	C,F,A,	48004962(-)	`CCTGCG/ACTGTC`	2	/A	syn¹ ref¹		26	MN NS EA NA WA	3718
rs1879835^1,2	C,F,A,	48007785(+)	`AAATTG/TTATTT`	1	--	int¹		8	NS NA EA WA	342
rs1879836^1,2	C,F,A,H,	48007859(+)	`TTCTGA/GAGTGG`	1	--	int¹		19	NS EA NA WA	1280
rs2037483^1,2	C,F,A,H,	48005122(+)	`AGCCTG/ACACCT`	1	--	int¹		12	NS NA MN EA	898
rs2037484^1,2	C,F,A,H,	48005250(+)	`AGGAAG/AAGTGA`	1	--	int¹ trp³		17	NS EA NA WA	1192
rs2037485^1,2	C,F,A,	48005983(+)	`GAATGT/CTGGGA`	1	--	int¹		9	NS EA NA WA	2034
rs2037486^1,2	C,F,A,H,	48006059(+)	`CAGAAG/AGTCCC`	1	--	int¹		11	NS EA NA WA	690

About this table

HapMap Linkage Disequilibrium images for HUS1 (up to first 250kb)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for HUS1: --

QIAGEN SeqTarget long-range PCR primers for resequencing HUS1

(in which this Gene is Involved, According to OMIM, UniProtKB, Novoseek, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, LSDB, HGMD, GAD, HuGE Navigator, and/or TGDB.)
About This Section

HUS1 for disorders About GeneDecksing

OMIM: 603760

2 Novoseek disease relationships for HUS1 gene

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
tumors	0	2	18156970 (2)
cancer	0	1	18156970 (1)

About this table

Human Genome Epidemiology (HuGE) Navigator: HUS1 (7 documents)

Export disorders and mutations for HUS1 gene to outside databases

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶UniProtKB/TrEMBL, and/or ⁷Novoseek)
About This Section

10/68 PubMed articles for HUS1 gene, integrated from 7 sources (see all 68):
(articles sorted by number of sources associating them with HUS1)

cDNA cloning and gene mapping of human homologs for Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of homologs from mouse, Caenorhabditis elegans, and Drosophila melanogaster. (PubMed id 9878245)^{1, 2, 3, 7} Dean F.B.... O'Donnell M. (1998)
Hus1p, a conserved fission yeast checkpoint protein, interacts with Rad1p and is phosphorylated in response to DNA damage. (PubMed id 9524127)^{1, 2, 3} Kostrub C.F.... Enoch T. (1998)
The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9. (PubMed id 10884395)^{1, 2, 7} Rauen M.... Karnitz L.M. (2000)
HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins. (PubMed id 10846170)^{1, 2, 7} Cai R.L.... Cohen D. (2000)
PCNA interacts with hHus1/hRad9 in response to DNA damage and replication inhibition. (PubMed id 11077446)^{1, 2, 7} Komatsu K.... Wang H.-G. (2000)
The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1. (PubMed id 10359610)^{1, 2, 7} St Onge R.P.... Davey S. (1999)
The J domain of Tpr2 regulates its interaction with the proapoptotic and cell-cycle checkpoint protein, Rad9. (PubMed id 11573955)^{1, 2, 7} Xiang S.L....Yamamoto K.C. (2001)
Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells. (PubMed id 15897895)^{1, 2, 7} Wu X.... Zou Y. (2005)
The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair. (PubMed id 15314187)^{1, 2, 7} Toueille M....Huebscher U. (2004)
The human checkpoint sensor and alternative DNA clamp Rad9-Rad1-Hus1 modulates the activity of DNA ligase I, a component of the long-patch base excision repair machinery. (PubMed id 15871698)^{1, 2} Smirnova E.... Huebscher U. (2005)

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Entrez Gene: 3364	HGNC: 5309	AceView: HUS1	Ensembl:ENSG00000136273	euGenes: HUgn3364
ECgene: HUS1	H-InvDB: HUS1

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