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TMEM222 Gene

protein-coding   GIFtS: 42

GC01P025904
transmembrane protein 222
(Previous name: chromosome 1 open reading frame 160 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: C1orf160)
Products    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
About This Section

Aliases & Descriptions
transmembrane protein 2221 2     OTTHUMP000000046372
C1orf1602 3     OTTHUMP000001972602
chromosome 1 open reading frame 1601     OTTHUMP000000046362
DKFZP564D04781     MGC1110022
DKFZp564D04782     

External Ids:    HGNC: 253631   Entrez Gene: 840652   Ensembl: ENSG000001865017   UniProtKB: Q9H0R33   

Export aliases for TMEM222 gene to outside databases

Previous GC identifers: GC01P027523 GC01P027648


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Gene Wiki entry for TMEM222 (C1orf160)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 60), Regulatory elements and Epigenetics data according to Qiagen and/or SABiosciences)
About This Section

Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for TMEM222
         Other transcription factors

   Search SABiosciences Chromatin IP Primers for TMEM222

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays for TMEM222 


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1p36.11   Ensembl cytogenetic band:  1p36.11   HGNC cytogenetic band: 1p36.11

TMEM222 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
TMEM222 gene location

GeneLoc gene densities for chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01P025904:  view genomic region     (about GC identifiers)

Start:
25,904,621 bp from pter
End:
25,918,879 bp from pter
Size:
14,259 bases
Orientation:
plus strand

1 alternative location:
Chr1+ 27,648,636-27,662,891     
RefSeq DNA sequence:
NC_000001.10  NT_004610.19  

(According to 1UniProtKB, neXtProt, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Millipore, Sigma-Aldrich, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, and/or ProSpec,
Biochemical Assays by Millipore, Sigma-Aldrich, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Dec 2010 and Entrez Gene, Antibodies by Millipore, Sigma-Aldrich, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, and/or Epitomics)
About This Section

UniProtKB/Swiss-Prot: TM222_HUMAN, Q9H0R3 (See protein sequence)
Recommended Name: Transmembrane protein 222  
Size: 208 amino acids; 23230 Da
Subcellular location: Membrane; Multi-pass membrane protein (Potential)
Sequence caution: Sequence=CAB66618.1; Type=Erroneous initiation;
Secondary accessions: Q53HD8 Q5FVE9
Alternative splicing: 2 isoforms:  Q9H0R3-1   Q9H0R3-2   

Explore the universe of human proteins at neXtProt for TMEM222: NX_Q9H0R3 

REFSEQ proteins: NP_115501.2  

ENSEMBL proteins: 
ENSP00000417853 ENSP00000419292 ENSP00000417720 ENSP00000363189 ENSP00000417603 ENSP00000363183 
ENSP00000419528 ENSP00000363186 ENSP00000419878 


Human Recombinant Proteins 
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
OriGene Purified Protein: TMEM222
OriGene Protein Over-expression Lysate: TMEM222
GenScript Custom Purified and Recombinant Proteins Services for TMEM222 
Novus Biologicals Lysate for TMEM222
Browse Sino Biological Recombinant Proteins
Browse ProSpec Recombinant Proteins

2 Gene Ontology (GO) cellular component terms (GO ID links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0016020 membrane IEA--
GO:0016021 integral to membrane IEA--
About this table

TMEM222 for ontologies           About GeneDecksing



Antibodies for TMEM222: 
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Browse OriGene Antibodies
GenScript Custom Superior Antibodies Services for TMEM222 
Browse antibodies at Epitomics

Assays for TMEM222: 
Browse Kits and Assays available from Millipore
Browse ELISAs at Sigma-Aldrich
OriGene Custom Immunoassay Development 
Browse OriGene Fluorogenic Cell Assay Kits 
Browse R&D Systems for biochemical assays
GenScript Custom Assay Services for TMEM222 
Browse Enzo Life Sciences for kits & assays
Uscn ELISAs and CLIAs for TMEM222 


(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

TMEM222 for domains           About GeneDecksing

1 InterPro domain/family:
 IPR008496 DUF778

Graphical View of Domain Structure for InterPro Entry Q9H0R3

ProtoNet protein and cluster: Q9H0R3


(According to UniProtKB, IUBMB,and/or Genatlas, Animal models from MGI Dec 24 2010,
shRNA from OriGene, Sigma-Aldrich, RNAi from Millipore, siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich, microRNA from Sigma-Aldrich, Qiagen, SABiosciences, Clones from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, Cell Lines from GenScript, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene.)
About This Section

Inhib.
RNA:
    
Browse for Gene Knock-down Tools from Millipore
Browse Nano Scale siRNA at Sigma-Aldrich
Sigma-Aldrich esiRNA and siRNA for TMEM222
Sigma-Aldrich shRNA for TMEM222
OriGene 29mer shRNA kits in GFP-retroviral vector: TMEM222
OriGene shRNA RFP: TMEM222
OriGene basic RS shRNA: TMEM222
OriGene siRNA: TMEM222
QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of TMEM222 
miRNA:Sigma-Aldrich microRNA Mimics for TMEM222
Browse microRNA target validation systems at Sigma-Aldrich 
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of TMEM222 
1 SABiosciences Assays for microRNA that regulate TMEM222:
hsa-miR-1827

Gene
Editing:
Browse CompoZr Knockout ZFN at Sigma-Aldrich 

Clones:Browse Clones for the Expression of Recombinant Proteins Available from Millipore
Browse iPSC Reprogramming Factors at Sigma-Aldrich
OriGene GFP tagged cDNA clones in CMV expression vector: TMEM222
OriGene Myc/DDK tagged cDNA clones in CMV expression vector: TMEM222
OriGene untagged cDNA clones in CMV expression vector (see all 2): TMEM222
OriGene 3'-UTR clone: TMEM222 
Browse MicroRNA Expression Plasmids 
GenScript Custom cDNA clone Services for TMEM222 
Browse Sino Biological Human cDNA Clones

Cell
Lines:
  
GenScript Custom overexpressing Cell Line Services for TMEM222 

1 Gene Ontology (GO) molecular function term (GO ID links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005515 protein binding IPI14667819
About this table

TMEM222 for ontologies           About GeneDecksing


(Pathways according to Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB, Sets of similar genes according to GeneDecks, PCR Arrays from SABiosciences, Proteins Network according to SABiosciences, Sigma-Aldrich, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Dec 2010 via Entrez Gene).
About This Section

    SABiosciences Custom Pathway-Focused PCR Arrays for TMEM222 

    Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for TMEM222

4 Interacting proteins for TMEM222 (Q9H0R31, 2) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
ABHD16AO958701, 2EBI-347385,EBI-348517 MINT-62701 MINT-62777 MINT-62603
BAT5O958701, 2
ENSG00000206403O958701, 2
PRSS23O950842MINT-63262
About this table

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section
Browse Small Molecules at Sigma-Aldrich
Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for TMEM222


(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 228 Homo sapiens; Dec 8 2010) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Millipore,
siRNAs from Sigma-Aldrich, OriGene, Qiagen, Super-pooled esiRNAs from Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene, microRNA from Sigma-Aldrich, Qiagen, SABiosciences,
Tagged/untagged cDNA clones from OriGene, Sigma-Aldrich, GenScript, Primers from OriGene and/or SABiosciences)
About This Section

Inhib.
RNA:
     
Browse for Gene Knock-down Tools from Millipore
Browse Nano Scale siRNA at Sigma-Aldrich
Sigma-Aldrich esiRNA and siRNA for TMEM222
Sigma-Aldrich shRNA for TMEM222
OriGene 29mer shRNA kits in GFP-retroviral vector: TMEM222
OriGene shRNA RFP: TMEM222
OriGene basic RS shRNA: TMEM222
OriGene siRNA: TMEM222
QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of TMEM222 
miRNA: Sigma-Aldrich microRNA Mimics for TMEM222
Browse microRNA target validation systems at Sigma-Aldrich 
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of TMEM222 
1 SABiosciences Assays for microRNA that regulate TMEM222:
hsa-miR-1827
Clones: Browse FLAG tag genes at Sigma-Aldrich
OriGene GFP tagged cDNA clones in CMV expression vector: TMEM222
OriGene Myc/DDK tagged cDNA clones in CMV expression vector: TMEM222
OriGene untagged cDNA clones in CMV expression vector (see all 2): TMEM222
OriGene 3'-UTR Clone: TMEM222 
Browse OriGene MicroRNA Expression Plasmids 
GenScript Custom cDNA clone Services for TMEM222 
Primers: OriGene genome-wide validated SYBR primer pairs: TMEM222
Browse OriGene validated miRNA SYBR primer pairs 
SABiosciences RT2 qPCR Primer Assay for TMEM222: PPH16886A

REFSEQ mRNAs for TMEM222 gene: 

NM_032125.2  

Additional cDNA sequence: 

AF251699.1 AK222642.1 AK303821.1 AK307077.1 AK313110.1 AL136683.1 BC011579.1 BC090039.1 
CR590629.1 CR591696.1 CR593094.1 CR603097.1 CR603432.1 CR603896.1 CR605192.1 CR605732.1 
CR607480.1 CR608798.1 CR609586.1 CR612028.1 CR615465.1 CR615612.1 CR623375.1 

10 DOTS entries:

DT.216024  DT.92436138  DT.100818591  DT.100818596  DT.100818598  DT.95155604  DT.75118198  DT.100818590 
DT.405134  DT.95155606 

24/300 AceView cDNA sequences (see all 300):

BM994918 BI859484 BM547871 T34456 CR623375 CF126155 BM465930 R46487 
BQ917627 AA852345 BG231858 BQ927627 BM673579 BQ054301 BM550098 CD675760 
BQ716961 AI129072 CR603097 BQ883835 AI566224 AK123218 BP346530 AA594674 

Unigene Cluster for TMEM222:

Transmembrane protein 222
Hs.469171  [show with all ESTs]
Unigene Representative Sequence: BC090039


GeneLoc Exon Structure

5/7 Alternative Splicing Database (ASD) splice patterns (SP) for TMEM222 (see all 7)

ExUns: 1a · 1b · 1c · 1d · 1e ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8 ^ 9a · 9b · 9c
SP1:                                                  -     -                 -     -                     
SP2:                                      -           -     -                 -                           
SP3:                                      -           -     -                 -     -                     
SP4:                                      -           -     -                                             
SP5:                                                                          -     -                     

About this scheme

ECgene alternative splicing isoforms for TMEM222
11 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000470223 ENST00000464813 ENST00000498220 ENST00000486082 ENST00000374076
ENST00000478104 ENST00000374070 ENST00000464720(uc001bnt.3) ENST00000374073(uc001bnr.3 uc001bns.3 uc001bnu.3)
ENST00000471456 ENST00000466759

(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 228 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Primers from OriGene and/or SABiosciences )
About This Section

TMEM222 expression in normal and diseased human tissues

1  / 2

6 probe-sets matching TMEM222 gene

Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
52078_at2 U95-B 1 1.00 1.00 0.84 0.97 -- -- -- -- --
57275_at2 U95-C 1 0.88 1.00 0.71 1.03 -- -- -- -- --
52078_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --
221512_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --
52078_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
221512_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
About this table

TMEM222 for expression           About GeneDecksing

Data from Genenote  (Publications) and GNF BioGPS
    About these images
TMEM222 gene expression
TMEM222 gene electronic northern expression
TMEM222 gene sage expression
About these images

CGAP SAGE TAG: CTGGTGATGG

SOURCE GeneReport for Unigene cluster: Hs.469171
Primers: OriGene genome-wide validated SYBR primer pairs: TMEM222
Browse OriGene validated miRNA SYBR primer pairs 
SABiosciences RT2 qPCR Primer Assay for TMEM222: PPH16886A
    SABiosciences Custom PCR Arrays for TMEM222 


(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Dec 24 2010, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section

Orthologs for TMEM222 gene from 5/15 species (see all 15)
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
TMEM2221   -- transmembrane protein 222 91.19(n)
95.19(a)
610172  XM_847412.1  XP_852505.1 
chimpanzee
(Pan troglodytes)
TMEM2221   -- transmembrane protein 222 95.92(n)
95.21(a)
456672  XM_513242.2  XP_513242.2 
cow
(Bos taurus)
TMEM2221   -- transmembrane protein 222 93.75(n)
98.21(a)
614627  NM_001035451.1  NP_001030528.1 
rat
(Rattus norvegicus)
Tmem2221   -- transmembrane protein 222 89.9(n)
95.19(a)
313021  XM_232731.4  XP_232731.3 
mouse
(Mus musculus)
Tmem2221 , 5 4 (66.25 cM)5
transmembrane protein 2221, 5 89.24(n)1
96.2(a)1
521741  NM_025667.21  NP_079943.11 
 AA4093415  AI8478635  (see all 16)
About this table        Species with no ortholog for TMEM222

ENSEMBL Gene Tree for TMEM222 (if available)

(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
  --

(SNPs according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Resequencing Primers from Qiagen)
About This Section

Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for TMEM222
     1 CNV: 48306
Search QIAGEN SeqTarget long-range PCR primers for resequencing for TMEM222 

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, LSDB, HGMD, GAD, HuGE Navigator, and/or TGDB.)
About This Section
  --

(Possibly Related Articles in Doctor's Guide)
About This Section
  --

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6UniProtKB/TrEMBL, and/or 7Novoseek)
About This Section

10 PubMed articles for TMEM222 gene integrated from 7 sources:
(articles sorted by number of sources associating them with TMEM222)
  1. Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. (PubMed id 11230166)1, 2, 3 Wiemann S.... Poustka A. (2001)
  2. The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)1, 2 Gregory S.G.... Bentley D.R. (2006)
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
  4. A human protein-protein interaction network: a resource for annotating the proteome. (PubMed id 16169070)1 Stelzl U.... Wanker E.E. (2005)
  5. Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions. (PubMed id 15342556)1 Suzuki Y.... Sugano S. (2004)
  6. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1 Ota T.... Sugano S. (2004)
  7. Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region. (PubMed id 14667819)1 Lehner B....Sanderson C.M. (2004)
  8. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
  9. Construction and characterization of a full length-enriched and a 5'- end-enriched cDNA library. (PubMed id 9373149)1 Suzuki Y.... Sugano S. (1997)
  10. Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. (PubMed id 8125298)1 Maruyama K. and Sugano S. (1994)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section
 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section
Entrez Gene: 84065 HGNC: 25363 AceView: DKFZP564D0478 Ensembl:ENSG00000186501 euGenes: HUgn84065
ECgene: TMEM222 H-InvDB: TMEM222

(According to HUGE)
About This Section
  --

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
  --

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from XenneX, Inc.)
About This Section
Patent Information for TMEM222 gene:
Search GeneIP for patents involving TMEM222

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays by Millipore, Sigma-Aldrich, R&D Systems, Qiagen, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Epitomics, ProSpec, Uscn,
Clones available from Millipore, Sigma-Aldrich, OriGene, GenScript, Sino Biological, PCR Arrays from SABiosciences, Drugs and/or compounds by Sigma-Aldrich, Tocris Bioscience, and/or Enzo Life Sciences)
About This Section

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
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 Millipore Target Screening & Profiling Services

 Predesigned and custom siRNAs for TMEM222 Browse antibodies at Sigma-Aldrich
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 Lentivirus-delivered shRNAs for TMEM222 microRNA Mimics for TMEM222
 "Your Favorite Gene" Pathways and Molecular Interaction Networks Browse Nano Scale siRNA at Sigma-Aldrich
 Browse iPSC Reprogramming Factors at Sigma-Aldrich Browse small molecules at Sigma-Aldrich
 Browse FLAG tag genes at Sigma-Aldrich Browse ELISAs at Sigma-Aldrich
 Browse microRNA Target Validation Systems at Sigma-Aldrich Browse CompoZr Knockout ZFN at Sigma-Aldrich
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 QIAGEN Custom miScript Target Protector blocks miRNA-binding site of TMEM222
 Search QIAGEN SeqTarget long-range PCR primers for resequencing for TMEM222
 QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays for TMEM222
 QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing of TMEM222
 GenScript Custom Purified and Recombinant Proteins Services for TMEM222 GenScript Custom cDNA clones with any tag delivered in your preferred vector Services for TMEM222
 GenScript Custom Assay Services for TMEM222 GenScript Custom Superior Antibodies Services for TMEM222
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 ELISAs and CLIAs for TMEM222
       

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